• 1,103 Views
  • 722 Downloads
Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 37-46
doi:10.32527/2017/101424

Effect of Telmisartan on Ischemia Reperfusion Induced Testicular Injury in Rats

Marwa M. M. Refaie1, Seham A. Abdel-Gaber1, and Rehab K. Mohammed2

1Department of Pharmacology, Faculty of Medicine, Minia University, 61511, Minia, Egypt

2Department of Pathology, Faculty of Medicine, Minia University, 61511, Minia, Egypt.

Copyright © 2017 Marwa M. M. Refaie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Torsion/detorsion of the testis is considered as surgical urologic emergency that affects newborn, infant and adolescent males that leads to testicular ischemia-reperfusion (TIR) injury. TIR can lead to testicular necrosis with subfertility or infertility. We investigated the effect of angiotensin II receptor blocker Telmisartan (TEL) (10 mg/kg i.m. 30 min before the reperfusion) on experimental TIR injury in rats. Rats were divided into four groups. Sham operated control group, TEL treated control group, TIR non-treated group, TEL treated TIR group. Testicular injury was evaluated by testicular weight changes and serum testosterone level and confirmed by histopathology. Testicular content of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and nitric oxide (NO) were evaluated. Moreover; the testicular immunohistochemical expression of the inflammatory marker tumor necrosis factor-alpha (TNF-α) was evaluated. The study showed that TEL has a protective effect against TIR injury. The protective effect of TEL was associated with modulation of testicular weight, serum testosterone level and the histopathological changes. TEL corrected the testicular oxidative stress parameters MDA, GSH, SOD, and NO with reduction of the testicular inflammatory marker TNF-α immunoexpression. The protective effect of TEL in TIR was attributed, at least in part, to its antioxidant and anti-inflammatory characters.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 37-46
doi:10.32527/2017/101424

Effect of Telmisartan on Ischemia Reperfusion Induced Testicular Injury in Rats

Marwa M. M. Refaie1, Seham A. Abdel-Gaber1, and Rehab K. Mohammed2

1Department of Pharmacology, Faculty of Medicine, Minia University, 61511, Minia, Egypt

2Department of Pathology, Faculty of Medicine, Minia University, 61511, Minia, Egypt.

Copyright © 2017 Marwa M. M. Refaie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Torsion/detorsion of the testis is considered as surgical urologic emergency that affects newborn, infant and adolescent males that leads to testicular ischemia-reperfusion (TIR) injury. TIR can lead to testicular necrosis with subfertility or infertility. We investigated the effect of angiotensin II receptor blocker Telmisartan (TEL) (10 mg/kg i.m. 30 min before the reperfusion) on experimental TIR injury in rats. Rats were divided into four groups. Sham operated control group, TEL treated control group, TIR non-treated group, TEL treated TIR group. Testicular injury was evaluated by testicular weight changes and serum testosterone level and confirmed by histopathology. Testicular content of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and nitric oxide (NO) were evaluated. Moreover; the testicular immunohistochemical expression of the inflammatory marker tumor necrosis factor-alpha (TNF-α) was evaluated. The study showed that TEL has a protective effect against TIR injury. The protective effect of TEL was associated with modulation of testicular weight, serum testosterone level and the histopathological changes. TEL corrected the testicular oxidative stress parameters MDA, GSH, SOD, and NO with reduction of the testicular inflammatory marker TNF-α immunoexpression. The protective effect of TEL in TIR was attributed, at least in part, to its antioxidant and anti-inflammatory characters.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 37-46
doi:10.32527/2017/101424

Effect of Telmisartan on Ischemia Reperfusion Induced Testicular Injury in Rats

Marwa M. M. Refaie1, Seham A. Abdel-Gaber1, and Rehab K. Mohammed2

1Department of Pharmacology, Faculty of Medicine, Minia University, 61511, Minia, Egypt

2Department of Pathology, Faculty of Medicine, Minia University, 61511, Minia, Egypt.

Copyright © 2017 Marwa M. M. Refaie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite this Article

Marwa M. M. Refaie, Seham A. Abdel-Gaber, and Rehab K. Mohammed, “Effect of Telmisartan on Ischemia Reperfusion Induced Testicular Injury in Rats,” Egyptian Journal of Basic and Clinical Pharmacology, Vol. 7, Issue 2, pp. 37-46, 2017. doi:10.32527/2017/101424