Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 9-24
doi:10.32527/2017/101422
Evaluation of the Therapeutic Effects of Polyvinylpyrrolidone-Capped Silver Nanoparticles on the Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Rats
Abstract
Globally, hepatocellular carcinoma (HCC) is the second-ranked cause of cancer-related deaths. Induction of anti-angiogenesis and apoptosis are highly effective strategies for treating HCC. Emergence of the anti-angiogenic and apoptotic properties of silver nanoparticles (AgNP) is a developing policy in cancer therapy. The present study was conducted to evaluate the therapeutic effects of polyvinylpyrrolidone (PVP)-capped AgNPs on chemically-induced HCC in rats. Induction of HCC model in male albino Wistar rats (220-250 g) was initiated by a single intraperitoneal injection of diethylnitrosamine (DEN; 200 mg/kg), then after 2 weeks promoted intraperitoneally with carbon tetrachloride (CCl4) solution (CCl4/olive oil; 1:1; 1 ml/kg) three times weekly for 6 weeks. HCC was confirmed through significant reductions in survival rates, serum albumin, elevations in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alphafetoprotein (AFP) levels and hepatic vascular endothelial growth factors (VEGF), platelet-derived growth factors (PDGF), nitric oxide (NO), interleukin (Il)-4 and -8, and tumor necrosis factor-alpha (TNF-α) levels, in addition to the histopathological dysplastic changes. PVP-AgNPs (30, 125, and 300 mg/kg/day) were given through oral gavage for 28 days. PVP-AgNPs therapy showed significant amelioration of these injurious effects with enhancement in the hepatic caspases activities, translated histopathologically to a marked improvement in the hepatic architecture. PVP-AgNPs 125mg/kg/day regimens displayed the best therapeutic effects, which considered a therapeutic modality for HCC regarding their anti-inflammatory, antiangiogenic, and caspase-dependent apoptotic effects. Advanced targeted molecular studies for use of single or combined regimens of PVP-AgNPs with different chemotherapeutics are recommended.
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 9-24
doi:10.32527/2017/101422
Evaluation of the Therapeutic Effects of Polyvinylpyrrolidone-Capped Silver Nanoparticles on the Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Rats
Abstract
Globally, hepatocellular carcinoma (HCC) is the second-ranked cause of cancer-related deaths. Induction of anti-angiogenesis and apoptosis are highly effective strategies for treating HCC. Emergence of the anti-angiogenic and apoptotic properties of silver nanoparticles (AgNP) is a developing policy in cancer therapy. The present study was conducted to evaluate the therapeutic effects of polyvinylpyrrolidone (PVP)-capped AgNPs on chemically-induced HCC in rats. Induction of HCC model in male albino Wistar rats (220-250 g) was initiated by a single intraperitoneal injection of diethylnitrosamine (DEN; 200 mg/kg), then after 2 weeks promoted intraperitoneally with carbon tetrachloride (CCl4) solution (CCl4/olive oil; 1:1; 1 ml/kg) three times weekly for 6 weeks. HCC was confirmed through significant reductions in survival rates, serum albumin, elevations in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alphafetoprotein (AFP) levels and hepatic vascular endothelial growth factors (VEGF), platelet-derived growth factors (PDGF), nitric oxide (NO), interleukin (Il)-4 and -8, and tumor necrosis factor-alpha (TNF-α) levels, in addition to the histopathological dysplastic changes. PVP-AgNPs (30, 125, and 300 mg/kg/day) were given through oral gavage for 28 days. PVP-AgNPs therapy showed significant amelioration of these injurious effects with enhancement in the hepatic caspases activities, translated histopathologically to a marked improvement in the hepatic architecture. PVP-AgNPs 125mg/kg/day regimens displayed the best therapeutic effects, which considered a therapeutic modality for HCC regarding their anti-inflammatory, antiangiogenic, and caspase-dependent apoptotic effects. Advanced targeted molecular studies for use of single or combined regimens of PVP-AgNPs with different chemotherapeutics are recommended.
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 9-24
doi:10.32527/2017/101422
Evaluation of the Therapeutic Effects of Polyvinylpyrrolidone-Capped Silver Nanoparticles on the Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Rats
How to Cite this Article
Samah M. Elaidy, Amr Moghazy, and Mohamed K. El-Kherbetawy, “Evaluation of the Therapeutic Effects of Polyvinylpyrrolidone-Capped Silver Nanoparticles on the Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Rats,” Egyptian Journal of Basic and Clinical Pharmacology, Vol. 7, Issue 2, pp. 9-24, 2017. doi:10.32527/2017/101422.